WebMay 15, 2015 · "The first report of in vivo imaging using Gluc demonstrated sensitivity that is several orders of magnitude higher than the first generation luciferases, Firefly … WebFirefly Renilla Figure 3. Comparison of the linear ranges of firefly and Renilla luciferases. The DLRŽ Assay was performed with a mixture of purified firefly and Renilla luciferases prepared in PLB containing 1mg/ml gelatin. A Turner Designs Model 20e Luminometer was used to measure luminescence. As shown in this graph with the
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WebApr 14, 2015 · Luciferase (firefly luciferase (FL) and Renilla luciferase (RL)) activity in CHO-K1 cells cultured on the DNA–fibronectin–apatite composite layers (DF-Ap layers) immobilizing FL gene (Sample FL) and RL gene (Sample DF-RL). Both Samples FL and RL were adjacently placed in one well of a four-well chamber slide. The results shown are … WebJun 1, 2000 · Stable cell lines (CHO) transfected with either human corticotrophin releasing hormone 1 (hCRH1) receptors and a firefly luciferase reporter gene or hCRH2 and a Renilla luciferase reporter gene were created to provide different luciferase readouts for CRH1 and CRH2 receptors, respectively. Cells were combined for stimulation and … bobicraft historias
Secreted dual reporter assay with Gaussia luciferase and the red
WebFirefly and Renilla-Based Reporter Assays: One-step protocol, without cell lysis: Yes. Reporter molecule is naturally secreted into the media and can be assayed without lysing cells. No. 2–4 fold higher signal than Renilla or firefly assays: Yes. No. Multiple data points per well: Yes. Single well can be used for multiple time points, as ... WebThis kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene … WebpGL3basic luciferase and renilla_polyA: Firefly/Renilla: Insertion of 5' promoter/enhancer regions upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur: … bobicraft house